- In vitro and cell molecular biology techniques: radioligand binding assays, bioluminescent and fluorescent resonance energy transfer (BRET/FRET) and proximity ligation assays, protein purification techniques, enzymatic activity assays, PCR/qPCR, DNA cloning. Cell culture techniques, cell signaling pathway determinations as G protein activation, cAMP accumulation or ERK1/2 phosphorylation and fluorescent and confocal microscopy.

- Animal models: behavioral assays (B, C and D functions (FELASA)).

- Strong knowledge of standard bioinformatic data resources and scientific software such as GraFit, GraphPadPrism, ImageJ

The candidate will need to have at least 3 years of postdoctoral experience and to demonstrate expertise in studying allosteric interactions between receptors. Specifically, the candidate needs to have proficiency and in performing radiochemical assays for affinity determinations and BRET/FRET and proximity ligation assays for detecting protein-protein interactions applied to receptors. Moreover, she/he will need to demonstrate experience in performing assays to determine cell signaling pathway activations using BRET/FRET methods, label-free and HTRF assays, WesternBlot or immunoassays. She/he must have expertise in confocal microscopy, cell culture and in working with animal models.

The candidate must have publications in international journals on GPCRs in general, and in opioid receptors in particular.

Knowledge of Spanish and English is required.

Gross salary per year 16406,28

A candidate is required for the position of researcher postdoctoral fellow with at least 3 years of postdoctoral experience.

The candidate must demonstrate expertise in studying allosteric interactions between receptors. Specifically, the candidate needs to have proficiency and in performing radiochemical assays for affinity determinations and BRET/FRET and proximity ligation assays for detecting protein-protein interactions applied to receptors. Moreover, she/he will need to demonstrate experience in performing assays to determine cell signaling pathway activations using BRET/FRET methods, label-free and HTRF assays, WesternBlot or immunoassays. She/he must have expertise in confocal microscopy, cell culture and in working with animal models.

The candidate must have publications in international journals on GPCRs in general, and in opioid receptors in particular.

Knowledge of Spanish and English is required.

CV (50 points)

PhD similar to topic of the offer (10 points)

Motivation letter (20 points)

Pre- and post- doctoral experience in using the techniques needed for this project (20 points):   <3 years: maximun 5 points; 3-5 years: maximum 10 points; >5 years: maximum 20 points

Priority will be given to people with disabilities (Law 89/2015 of June 2, reserve of quota 2% in favour of people with disabilities in companies of 50 or more people).