Postdoc position available for research on new high-throughput imaging technology. The AG Karpf lab at the Institute of biomedical optics at the University of Luebeck, Germany, is developing one of the fastest two-photon microscopy techniques in the world. The spectro-temporal laser imaging by diffractive excitation (SLIDE) technique achieves frame-rates in excess of 1 kHz, i.e. about two orders of magnitude faster than conventional two-photon microscopes. The candidate will develop an imaging flow cytometer based on the SLIDE microscopy technique, capable of visualizing individual cells within thick tissue specimen. Using the deep penetration of multiphoton microscopy, this work will image cells at relevant spatio-temporal context, i.e. cellular sub-units like nuclei as well as the whole cellular context in tissue fragments. This will allow high-throughput analysis to develop new diagnostic tools. The work will involve researching suitable contrast agents for high sensitivity and specificity, as well innovation in three-dimensional imaging. The position is fully funded for a three year period and will be paid according to federal contractive salary TV-L E13 (please use online services like https://oeffentlicher-dienst.info and https://www.brutto-netto-rechner.info for estimates on your take-home salary).  

The research will be hosted in the biophotonic research group lead by Prof. Sebastian Nino Karpf at the Institute of biomedical optics (BMO) at the Universität zu Lübeck. The group explores the recently invented SLIDE technology to image biomedical research questions in the context of cell imaging and Imaging flow cytometry, intravital imaging using high-speed 4D microscopy and harmonic imaging by developing novel, advanced laser technology and imaging microscopes. A background in physics/optics/lasers/imaging/biomedical engineering or related subjects is desired. International exchange and collaborative, interdisciplinary research travels are actively promoted.

Please apply by sending a motivational letter, your current CV and your research track record. Come work in an interdisciplinary team and help build new tools for biomedical research. For more Info visit: https://www.bmo.uni-luebeck.de/en/research/ag-karpf.html or send an e-mail to Prof. Karpf.

Application deadline: March 31, 2025

Related Publications:

1,2

1. Karpf, S. et al. Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates. Nature communications11, 1–9 (2020).

2. Karpf, S. et al. Harmonic Imaging of Stem Cells in Whole Blood at GHz Pixel Rate. Small 2401472 (2024) doi:10.1002/smll.202401472.