Post-doctoral fellow (M/F) for the development of double objective configuration for super-resolution microscopy

The Nanobio team is developing new concepts to push back the limits of single-molecule localization microscopy. In particular, we have recently patented various concepts for localizing molecules in structured illumination inducing fluorescence modulation. The aim of this position is to develop a new optical configuration based on the use of two face-to-face objectives to create interferences along the optical axis, and thus encode the position of molecules along this axis with ultimate precision. Unlike 4pi microscopy, detection requires demodulation via just one of the objectives, and the image detected on the second objective can be used to extract spectral information. These new developments are based on a first patented proof-of-concept, and will be implemented as part of the TimeNanoLive ERC. The position may be extended for a further 12 months.

The person will contribute to various aspects :
- setting up the optical implementations of this new concept
- testing and validation of the new experimental set-up
- pilot/simulation programming
- characterisation of various samples
- writing articles
- taking part in conferences

This work will be carried out within ISMO (Unité Mixte CNRS / Université Paris-Saclay) as part of a European proejct ERC TimeNanolive ). The laboratory has an on-site culture room and various microscopes for single molecule localization microscopy.