PhD position (E13 TV-L, 65%) – Mechanobiology of vascular infections

The Bastounis lab in the Interfaculty Institute of Microbiology & Infection Medicine (Cluster of Excellence “Controlling Microbes to Fight Infections”, CMFI EXC 2124) at the University of Tübingen is looking to fill one PhD position (E13 TV-L, 65%) as part of a recently funded DFG funded project. The earliest starting date for this position will be on 01.07.2024. The position is funded for a period of three years. The available position focuses on deciphering the mechanobiological processes involved in vascular endothelial cell infection by food-borne facultative intracellular bacterial pathogen Listeria monocytogenes.

Specific research project

Endothelial cells that line the inner lumen of our vessels are constantly exposed to shear stresses due to blood flow and are experiencing alterations in the stiffness of the extracellular matrix (ECM) on which they reside (Bastounis et al., 2019). Changes in both these mechanical cues contribute to multiple pathologies including atherosclerosis, and often correlate with in- creased susceptibility to bacterial infection (Bastounis et al., 2018). However, most clinical studies on these correlations lack causality and are rather observational in nature. Using a multi-well impinging flow jet to impart varying shear stresses and gradients into endothelial cells in culture residing on varying stiffness ECMs we will investigate how those alone and in synergy impact direct adhesion and uptake of pathogenic intracellular bacteria, as well as their ability to spread from cell to cell. The goal of the project is to discover how shear flows impact host cell mechanotransduction and tension, thus facilitating or obstructing intracellular bacterial pathogen dissemination through endothelia.

For further information about the position, please contact Effie Bastounis by e-mail at effie.bastounis@uni-tuebingen.de, and visit our website https://www.bastounislab.org. Ap- plications with a short cover/motivation letter, CV/biosketch, diploma(s) and two contacts for references should be sent via email to effie.bastounis@uni-tuebingen.de.

The University aims to increase the proportion of women in research and teaching and urges suitably qualified women scientists to apply. Qualified international researchers are expressly invited to apply. Disabled persons with equal aptitude will be given preferential consideration.