Ref. HR23-00749; PerMedFH-BPD1

Generic Scientific Area: Health or Biological Sciences

Admission Requirements:

Applications meeting the following requirements will be considered:

• Ph.D. completed less than 3 years from the application deadline or obtain a doctoral degree by March 31, 2024 (mandatory);
• At least 3 years' experience in molecular biology in the field of genetic dyslipidemias.
• At least 2 years' experience in high-throughput microscopy / automated cell imaging.
• Availability for a 3-6 month internship in Helsinki, Finland

Preferred Requirements:

1. Previous experience in the field of familial dyslipidemia;
2. Previous experience in isolating lymphocytes from peripheral blood samples;
3. RNA extraction from lymphocytes;
4. Previous experience in cell culture;
5. Previous experience in in vitro mutagenesis;
6. Previous experience in flow cytometry;
7. Good knowledge of English (written and oral);
8. Good interpersonal, communication and teamwork skills;
9. Immediate availability.

Monthly maintenance subsidy amount: The monthly value of the fellowship will correspond to the candidate's degree, according to the current value of fellowships awarded by the “Fundação para a Ciência e Tecnologia” (https://www.fct.pt/wp-content/uploads/2023/02/Tabela-de-Valores-SMM_2023.pdf ). To this value, the Voluntary Social Security amount (1st stage) is added, with Accident Insurance being paid.

• Ph.D. completed less than 3 years from the application deadline or obtain a doctoral degree by March 31, 2024 (mandatory);
• At least 3 years' experience in molecular biology in the field of genetic dyslipidemias.
• At least 2 years' experience in high-throughput microscopy / automated cell imaging.
• Availability for a 3-6 month internship in Helsinki, Finland

Candidates who do not meet the previously mentioned criteria will be excluded. Candidates who do not submit a graduation certificate will also be excluded. The evaluation will focus on the assessment of academic background and professional experience in the areas mentioned above. The evaluation process will include two phases. In the first phase, the CV and the candidate's motivation will be evaluated. Subsequently, candidates who, in this first evaluation, achieve a score not lower than 12 points on a scale of 0 to 20 points will be called for a knowledge and skills assessment interview. The weighting for the final evaluation will be 50% for the curriculum evaluation and 50% for the interview. Candidates will be classified, and the candidate with the highest score will be accepted. In case of the first selected candidate's inability to accept the fellowship, the option will be the second-ranked candidate (and so on) according to the list ordered by the Jury of the recruitment process. However, the jury reserves the right to decide that none of the available candidates meets the minimum requirements to fill the vacant position, in which case a new recruitment process must be carried out.

Work Plan:

1. Isolation of lymphocytes from peripheral blood samples;
2. RNA extraction from lymphocytes and cell lines;
3. Splicing studies by minigenes;
4. Maintenance of cell cultures;
5. In vitro mutagenesis;
6. Flow cytometry assays (FACS);
7. High-throughput microscopy/automated cell imaging assays