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Open position for a ERC funded POSTDOCTORAL position (Mammalian cell culture expertise) at UNINA

22 Nov 2022

Job Information

Organisation/Company
University of Naples Federico II
Department
Department of Chemical Sciences
Research Field
Technology » Biotechnology
Biological sciences » Biology
Pharmacological sciences » Clinical pharmacology
Medical sciences » Other
Pharmacological sciences » Other
Biological sciences » Other
Technology » Other
Biological sciences » Laboratory animal science
Pharmacological sciences » Clinical pharmacology
Medical sciences » Other
Researcher Profile
Recognised Researcher (R2)
Established Researcher (R3)
Country
Italy
Application Deadline
Type of Contract
Other
Job Status
Full-time
Hours Per Week
40
Is the job funded through the EU Research Framework Programme?
H2020 / ERC
Is the Job related to staff position within a Research Infrastructure?
No

Offer Description

University of Naples Federico II in Italy (UNINA), hosted by the Department of Chemical Sciences, is taking applications for a two-year post-doctoral research position in an European Research Council (ERC) funded project (DEBUGGING LPS) investigating the impact of gut microbiota lipopolysaccharides (LPS) and synthetic derivatives on human in vitro immune and non-immune cellular models.

The specific objectives of the research activity are: a) To delineate the immunological properties of specific LPS isolated from gut bacteria and synthetic analogues in human immune cell models. b) To investigate the molecular mechanisms involved in the activation and development of a specific immunological outcome. c) To evaluate the impact of these molecules in in vitro cell models mimicking human intestine, both in health and disease. d) To identify potential candidates of therapeutic value, capable of activating “protective” signaling pathways or able to dampen pro-inflammatory ones.

Requirements

Research Field
Biological sciences » Biology
Education Level
PhD or equivalent
Research Field
Technology » Biotechnology
Education Level
PhD or equivalent
Research Field
Pharmacological sciences » Clinical pharmacology
Education Level
PhD or equivalent
Research Field
Medical sciences » Other
Education Level
PhD or equivalent
Research Field
Other
Education Level
Master Degree or equivalent
Skills/Qualifications

The highly motivated candidate should have taken his/her Ph.D. degree within the past 6 years in one of the following areas: Clinical and Experimental Medical Sciences, Molecular Medicine, Medicinal Chemistry, Biochemistry, Molecular Biology, Biology, Pharmacology, Biotechnology, and areas related to drug discovery or similar fields. 2-5 years of relevant experience in cell culture in academia or cell biology industry will be evaluated favorably.

The candidate must be interested in cellular immunology and biology techniques with skills in cell cultures, biochemical and immunological assays. He/She must be able to conduct research aimed at drug discovery and development, using biochemical and basic cellular immunology assays.

The ideal candidate must be capable of performing cell culture techniques: cell counts and other assays (e.g., mycoplasma testing) as needed to ensure the quality of cell preparations; plating and freezing storage of various cell lines (preferably: HEK, THP1, RAW 264.7, Caco2, HT29-MTX-E12, SHYH5Y); transfecting cells with the desired gene(s); stimulation and evaluation of the results by using these cell models; setting and use of co-culture system(s) (2D model) such as Caco2-RAW 264.7, Caco2-HT29-MTX-E12 cells and Caco2-SHSY5Y and monolayer differentiation of gut membrane.

The candidate should be able to perform: RNA and Protein extraction, SDS-PAGE, Western blot, Spectrophotometric protein quantification assays, immunohistochemical staining, ELISA, RT-PCR, TEER measurement; Nitric Oxide Colorimetric Assay; MTT vitality assay, fluorescent-activated cell sorting (FACS), and confocal microscopy. Basic knowledge of 3D organoid cultures will be considered favorably.

Specific Requirements

This role requires an individual with a proven track record in research, combined with the ability to manage a team and resolve technical issues.

We will consider as an advantage:

• Ability to work on tight deadlines with a sense of urgency

• Experience with Standard Operating Procedures when performing lab functions, maintaining paperwork and batch records, but also working independently and collaboratively as needed to accomplish goals.

• Strong problem-solving attitude.

• Ability to disseminate scientific results (papers, conferences,..)

Languages
ENGLISH
Level
Good
Research Field
Biological sciences » BiologyBiological sciences » Other
Years of Research Experience
4 - 10
Research Field
Biological sciences » OtherBiological sciences » Other
Years of Research Experience
4 - 10
Research Field
Technology » BiotechnologyTechnology » Other
Years of Research Experience
4 - 10
Research Field
Pharmacological sciences » Clinical pharmacologyPharmacological sciences » Other
Years of Research Experience
4 - 10
Research Field
Pharmacological sciences » Clinical pharmacologyPharmacological sciences » Other
Years of Research Experience
4 - 10
Research Field
Medical sciences » OtherMedical sciences » Other
Years of Research Experience
4 - 10

Additional Information

Additional comments

Submit your complete application by January 15th 2023 (cover letter, CV and 2 recommendation letters) to Prof. Flaviana Di Lorenzo (flaviana.dilorenzo@unina.it).

Work Location(s)

Number of offers available
1
Company/Institute
University of Naples Federico II
Country
Italy
State/Province
Italy
City
Naples
Postal Code
80126
Street
via cinthia 4
Geofield

Where to apply

E-mail
flaviana.dilorenzo@unina.it

Contact

State/Province
Italy
City
Naples
Website
Street
via cinthia 4
Postal Code
80126
E-Mail
flaviana.dilorenzo@unina.it