22/06/2022
The Human Resources Strategy for Researchers

PhD project - imaging plankton interaction H/F

This job offer has expired


  • ORGANISATION/COMPANY
    CNRS
  • RESEARCH FIELD
    Biological sciences
    Environmental science
  • RESEARCHER PROFILE
    First Stage Researcher (R1)
  • APPLICATION DEADLINE
    13/07/2022 23:59 - Europe/Brussels
  • LOCATION
    France › GRENOBLE
  • TYPE OF CONTRACT
    Temporary
  • JOB STATUS
    Full-time
  • HOURS PER WEEK
    35
  • OFFER STARTING DATE
    01/10/2022

OFFER DESCRIPTION

The candidate will be part of the Photosymbiosis team (photosymbiosis.com) of the LPCV -laboratory of Cellular and Plant Physiology- (UMR 5168 - CNRS -CEA - Univ. Grenoble Alpes - INRA) within the IRIG institute of the CEA in Grenoble. The projects of the Photosymbiosis team aim to better understand the functioning of interactions between unicellular organisms (photosymbiosis, parasitism) by combining subcellular microscopy approaches (nanoSIMS, Synchrotron X-Ray fluorescence, 3D electron microscopy). Locally, the project will be in close collaboration with the IRIG institute: INAC platform (IRIG) and the Institute of Structural Biology (IBS, Grenoble).

Missions : Host-parasite interactions are widespread in single-celled marine plankton. The main objective of the project will be to better understand the trophic strategies of the parasite during its intracellular development in the host (dinoflagellate) using subcellular imaging techniques (nanoSIMS coupled to isotope labeling and 3D electron microscopy) and metabolomics (lipidomes). Moreover, to better understand the metabolism and infection mechanisms of the free form of the parasite, the second objective of the project will be to reveal the 3D ultrastructure of the dinospores released after infection with 3D electron microscopy approaches (FIB-SEM and cryo-tomography).
Activities :
- Culture of the microalgae (host) - parasite system in the laboratory
- Photo-physiological analyses (photosynthesis, carbon fixation)
- Incubation with stable isotopes coupled to nanoSIMS
- 3D electron microscopy (FIB-SEM and cryo-electron tomography)
- Image analysis (segmentation, 3D reconstruction)
- Communication through international meetings and workshops.

Background and skills :
- Training in biology
- Knowledge and interest in cellular interactions: mutualistic symbioses or parasitism
- Expertise in microalgae culture and physiology will be appreciated
- Knowledge and expertise in sample preparation for 3D electron microscopy (FIB-SEM, cryo-TEM), and image analysis for 3D reconstruction.
- Ability to work independently in an interdisciplinary and international environment (fluent in English), and to interact in a young and dynamic research group.

More Information

Required Research Experiences

  • RESEARCH FIELD
    Biological sciences
  • YEARS OF RESEARCH EXPERIENCE
    None
  • RESEARCH FIELD
    Environmental science
  • YEARS OF RESEARCH EXPERIENCE
    None

Offer Requirements

  • REQUIRED EDUCATION LEVEL
    Biological sciences: Master Degree or equivalent
    Environmental science: Master Degree or equivalent
  • REQUIRED LANGUAGES
    FRENCH: Basic
Work location(s)
1 position(s) available at
Laboratoire de physiologie cellulaire et végétale
France
GRENOBLE

EURAXESS offer ID: 803055
Posting organisation offer ID: 30088

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