07/06/2022
The Human Resources Strategy for Researchers

PhD Student (Male/Female)


  • ORGANISATION/COMPANY
    CNRS
  • RESEARCH FIELD
    Biological sciencesBiology
  • RESEARCHER PROFILE
    First Stage Researcher (R1)
  • APPLICATION DEADLINE
    28/06/2022 23:59 - Europe/Brussels
  • LOCATION
    France › GIF SUR YVETTE
  • TYPE OF CONTRACT
    Temporary
  • JOB STATUS
    Full-time
  • HOURS PER WEEK
    35
  • OFFER STARTING DATE
    01/09/2022

OFFER DESCRIPTION

Saclay, located until 2019 on the Gif and Saclay sites. The unit has an average staff of nearly 650 people divided between 5 scientific departments, 69 research teams, 14 high-level technological platforms and 11 support services (Steering, HR, Finance, H&S, Logistics and Technical, Partnerships) and support services (Platforms operation, Computing and scientific calculation, Laundries, Greenhouses and animal house). Its average annual budget is about 22 M€ from its supervisory bodies and nearly 180 live research contracts (Europe, ANR, local authorities, industry, etc.)
The student will join the "Evolution and Maintenance of Circular Chromosomes" (EMC2) team of the Department of Genome Biology, headed by François-Xavier Barre. He will be supervised by François-Xavier Barre and James Provan.

Exploring the functionality of Vibrio cholerae chromosome dimer resolution sites
Most bacteria have circular chromosomes. Homologous recombination events between the two sister chromosomes resulting from the replication of a circular chromosome can lead to the formation of a chromosome dimer. Chromosome dimers are resolved by the addition of a crossing over between the 2 copies of a specific site present on the dimer, dif, by two tyrosine recombinases, XerC and XerD. The absence of this system leads to the formation of filamentous cells and a loss of viability. Mobile elements are able to exploit the Xer machinery to integrate into their host genome. This is the case for CTXφ, a lysogenic filamentous phage from Vibrio cholerae, whose genome contains the genes encoding cholera toxin. There are several variants of CTXφ. The current variants integrate into the dif site of the larger of the 2 V. cholerae chromosomes, dif1. However, it has been reported that environmental strains of V. cholerae carry a defective dif1 site, dif1AT, which does not allow resolution of chromosome dimers or integration of CTXφ. The lysogenic conversion of these strains by CTXφ was proposed to depend on the prior integration of another mobile Xer-operating element, the satellite phage TLCφ, which leads to the replacement of dif1AT by a functional site, dif1GC. This hypothesis could explain why one or more copies of TLCφ are found before CTXφ in the genome of clinical strains.
The objective of the thesis project is to explore the functionality of the different dif variants found in the genome of Vibrio cholerae strains and to study how TLCφ participates in the integration of CTXφ.

More Information

Required Research Experiences

  • RESEARCH FIELD
    Biological sciencesBiology
  • YEARS OF RESEARCH EXPERIENCE
    None

Offer Requirements

  • REQUIRED EDUCATION LEVEL
    Biological sciences: Master Degree or equivalent
  • REQUIRED LANGUAGES
    FRENCH: Basic

Map Information

Job Work Location Personal Assistance locations
Work location(s)
1 position(s) available at
Institut de Biologie Intégrative de la Cellule
France
GIF SUR YVETTE

EURAXESS offer ID: 795959
Posting organisation offer ID: 29609

Disclaimer:

The responsibility for the jobs published on this website, including the job description, lies entirely with the publishing institutions. The application is handled uniquely by the employer, who is also fully responsible for the recruitment and selection processes.

 

Please contact support@euraxess.org if you wish to download all jobs in XML.