25/06/2021
The Human Resources Strategy for Researchers
European Research Council

PhD Student M / F: Study of cytoplasmic crowding during antifungal drug tolerance

This job offer has expired


  • ORGANISATION/COMPANY
    CNRS
  • RESEARCH FIELD
    Biological sciencesBiology
  • RESEARCHER PROFILE
    First Stage Researcher (R1)
  • APPLICATION DEADLINE
    06/08/2021 23:59 - Europe/Brussels
  • LOCATION
    France › NICE
  • TYPE OF CONTRACT
    Temporary
  • JOB STATUS
    Full-time
  • HOURS PER WEEK
    35
  • OFFER STARTING DATE
    15/09/2021
  • EU RESEARCH FRAMEWORK PROGRAMME
    H2020 / ERC

OFFER DESCRIPTION

The Valrose Institute of Biology (28 teams; 300 people; 25 nationalities) is an international institute (English is the working language) with a very rich scientific environment. The iBV provides cutting-edge technological infrastructures and platforms, with a collaborative and lively atmosphere in a very attractive city / region. (ibv.unice.fr).

The candidate will take advantage of a live-cell microscopy method, that has been recently established in Candida albicans, to quantitate cytoplasmic crowding in this opportunistic human pathogenic fungus, using a variety of molecular and cellular approaches.

A 3-year PhD fellowship is available in the Polarized growth in yeast group at the Institute of Biology Valrose (iBV) at the University of Côte d'Azur in Nice, FR
(http://ibv.unice.fr/research-team/arkowitz/).

Our team studies the spatial and temporal regulation of filamentous growth using a range of state-of-the art microscopy approaches. Drug tolerance is generally described as microbe survival or growth above inhibitory concentrations and subpopulations of C. albicans can grow above minimal inhibitory drug concentrations. Tolerance, i.e. slow growth in presence of drug, is dependent on cell stress response pathways. Cytoplasmic crowding is intimately tied to protein abundance, and hence affected by stresses such as starvation, which are activated via amino acid sensing, and also likely to affect drug tolerance. The goal of this collaborative project is to determine the link between antifungal drug tolerance and cytoplasmic crowding at the single cell level. The project will take advantage of cutting-edge imaging approaches, molecular genetics and image analyses to investigate relationship between physical characteristics of the cytoplasm and antifungal tolerance in C. albicans cells and communities.

Objectives:
-Determine the heterogeneity of cytoplasmic crowding in a population of cells and whether it correlates with an antifungal drug tolerance.
-Determine the link between intracellular antifungal drug concentration and cytoplasmic crowding.
-Quantify cytoplasmic crowding in a range of stress response mutants and clinical isolates.

Candidates should have a Master's degree or equivalent in a relevant field.
We are seeking highly motivated candidates with a background in Cell Biology, Microbiology, Microscopy or Biophysics - previous experience in live cell imaging and/or image analyses is a plus. Motivation, enthusiasm, ability to communicate in English and interact with lab members are critical and applicants are encouraged to apply and contact Robert Arkowitz (arkowitz@unice.fr).

Selected publications
1. R. Abdul-Ganiyu, L. A. Venegas, X. Wang, C. Puerner, R. A. Arkowitz, B. K. Kay & D. E. Stone. Sci Signal, in press.
2. C. Puerner, N, Kukhaleishvili, D. Thomson, S. Schaub, X. Noblin, S. Seminara, M. Bassilana & R. A. Arkowitz. BMC Biol. 2020 18: 122. Actualité scientifique CNRS INSB: https://insb.cnrs.fr/fr/cnrsinfo/confinement-force-biophysique-de-linvas...
3. M. Bassilana, C. Puerner & R. A. Arkowitz. Curr Op Cell Biol. 2020 62:150.
4. R. A. Arkowitz & Bassilana M. F1000 Res. 2019 8.
5. P. M. Silva, C. Puerner, A. Seminara, M. Bassilana & R. A. Arkowitz. Cell Rep. 2019 28: 2231. Actualité scientifique CNRS INSB:
https://insb.cnrs.fr/fr/cnrsinfo/reinitialiser-la-polarite-cellulaire-so...
6. A. Weiner, F. Orange, S. Lacas-Gervais, K. Rechav, V. Ghugtyal, M. Bassilana & R. A. Arkowitz. Cell Microbiol. 2019 21: e12963. Selected by Faculty 1000 December 18, 2018. https://f1000.com/prime/734234273?bd=1&ui=206550
7. H. Labbaoui, S. Bogliolo, V. Ghugtyal, N. V. Solis, S. G. Filler, R. A. Arkowitz & M. Bassilana. PLoS Pathogens. 2017 13: e1006205.
8. V. Ghugtyal, R. Garcia-Rodas, A. Seminara, S. Schaub, M. Bassilana & R. A. Arkowitz. PNAS USA. 2015 112: 8644.
9. S. G. Martin & R. A. Arkowitz. FEMS Microbiol Rev. 2014 38: 228
10. V. Corvest, S. Bogliolo, P. Follette, R. A. Arkowitz & M. Bassilana. Mol. Microbiol. 2013 89: 626.
11. A. Vernay, S. Schaub, I. Guillas, M. Bassilana & R. A. Arkowitz. J Cell Biol. 2012 198: 711. Selected by Faculty 1000 March 19, 2013. http://f1000.com/prime/717988257.

More Information

Additional comments

There is some fliexibility with respect to the PhD candidates salary, as a function of qualifications and experience.

Web site for additional job details

Required Research Experiences

  • RESEARCH FIELD
    Biological sciences
  • YEARS OF RESEARCH EXPERIENCE
    None
  • RESEARCH FIELD
    Biological sciencesBiology
  • YEARS OF RESEARCH EXPERIENCE
    None

Offer Requirements

  • REQUIRED EDUCATION LEVEL
    Biological sciences: Master Degree or equivalent
  • REQUIRED LANGUAGES
    FRENCH: Basic
Work location(s)
1 position(s) available at
Institut de Biologie Valrose
France
NICE

EURAXESS offer ID: 656550
Posting organisation offer ID: 22507

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