19/03/2021

PhD in Microbiology

This job offer has expired


  • ORGANISATION/COMPANY
    Institute for Integrative Biology of the Cell
  • RESEARCH FIELD
    Biological sciencesBiology
  • RESEARCHER PROFILE
    First Stage Researcher (R1)
  • APPLICATION DEADLINE
    15/05/2021 23:00 - Europe/Brussels
  • LOCATION
    France › Orsay
  • TYPE OF CONTRACT
    Temporary
  • JOB STATUS
    Full-time
  • HOURS PER WEEK
    35
  • OFFER STARTING DATE
    01/10/2021

OFFER DESCRIPTION

We are looking for two candidates to start two Joint PhD research projects between the Université de Sherbrooke and the Université Paris-Saclay. These two projects aim to study the interactions between bacteriophages (i.e. viruses or phages) and the pathogenic bacterium Clostridioides difficile, which is the main cause of post-antibiotic diarrhoea and is one of the most problematic bacteria in hospitals.

The first project will focus on the study of an antiphage system of the "abortive infection" (Abi) type controlled by a riboswitch that has been identified in the genome of a prophage. This project will be carried out mainly at the Université Paris-Saclay under the supervision of Prof. Soutourina, but will include a significant portion (at least 1 year) of work at the Université de Sherbrooke in the laboratory of Prof. Fortier.

The second project will focus on the study of the binding specificity between phages and their bacterial receptor, more specifically the Surface-layer protein A (SlpA). This project will take place mainly at the Université de Sherbrooke in Quebec under the direction of Prof. Fortier, but will include a significant portion (at least 1 year) of work at the Université Paris-Saclay in the laboratory of Prof. Soutourina.

For both projects, the research activities will include:

  • Anaerobic culture of C. difficile and infections with bacteriophages in a biocontainment level 2 working environment
  • Molecular cloning (construction of plasmids and mutants of C. difficile using different genetic and molecular tools)
  • Protein expression in C. difficile
  • Phenotypic characterization of C. difficile (growth, toxin production, sporulation)
  • Gene and protein expression analysis (Northern blot, RNA-seq, RT-qPCR, Western blot, reporter gene fusion)

More Information

Offer Requirements

Skills/Qualifications

Candidates should have a Master 2, MSc or equivalent degree. Candidates should ideally have relevant experience in microbiology and/or molecular biology, have good communication skills, and be prepared to study abroad for several months. We are committed to diversity, equity and inclusion and all candidates will be evaluated and considered equally.

Work location(s)
2 position(s) available at
I2BC
France
Orsay
91405
Rue Gregor Mendel

EURAXESS offer ID: 617895

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