25/02/2021

PhD Position in the Project "Development of a dissolvable microcarrier for use in a therapeutic stem cell expansion process (Carrier 4TSC)"

This job offer has expired


  • ORGANISATION/COMPANY
    International Giessen Graduate Centre for the Life Sciences
  • RESEARCH FIELD
    Pharmacological sciencesPharmacy
  • RESEARCHER PROFILE
    First Stage Researcher (R1)
  • APPLICATION DEADLINE
    11/04/2021 23:00 - Europe/Athens
  • LOCATION
    Germany › Giessen
  • TYPE OF CONTRACT
    Temporary
  • JOB STATUS
    Part-time
  • HOURS PER WEEK
    20
  • OFFER STARTING DATE
    01/05/2021

OFFER DESCRIPTION

 

The application of MSC as cell therapeutics is a growing sector in health care. MSC therapeutics contain ~ 10^8 cells per dose, an amount that cannot solely be isolated from donor tissue. Thus, an in-vitro MSC expansion process is necessary and crucial, as it has a high impact on the quality of the final MSC product. The main goal of the project is the development of a novel dissolvable microcarrier that enables the adhesion and growth of mesenchymal stem cells (MSC) in a bioreactor process, and an efficient cell harvest while preserving the therapeutic potential of MSC. The porous, starch-based microcarriers will be optimized to ensure a mechanical support and a high surface-to-volume ratio when applied in a bioreactor. It will be dissolvable to enable a harvesting procedure that avoids a proteolytic detachment of the cells. A novel coating of the microcarriers will combine synthetic, bioactive peptides and glycosaminoglycans. The coating will be MSC-specific and will enable the adhesion and survival of the anchorage-dependent MSC in a serum-free cell culture medium, and support the preservation of their functionality. The microcarriers will be applied in a stirred-tank bioreactor. Process parameters promoting an optimal yield and MSC functionality will be identified and optimized in a design-of-experiments (DOE) approach. Physicochemical, cell-associated, and equipment-associated process parameters were investigated to determine critical ones. A limited fed batch model will be developed and then coupled with an online monitoring of the cell biomass via impedance spectroscopy. This process control strategy will be tested in various scenarios and for different MSC cell types. The novel microcarrier prototypes are tested in a real MSC expansion process and investigated regarding their sedimentation behaviour and their influence on mixing time and mass transfer processes, and analysed regarding the adhesion, growth, and the harvest of the MSC in the stirred tank reactor.

More Information

Benefits

The project is located in the cell culture group (group leader Dr.-Ing. Denise Salzig) at the Institute of Biopharmaceutical Engineering and Pharmaceutical Technology (Managing director Prof. Dr-Ing. Peter Czermak). The cell culture group has over 10 years of experience in stem cell process development, one of her major research foci. Further foci are the viral process development and the process development for recombinant protein production in insect cell lines. The Institute is one of the most successful ones at THM in the acquisition of third-party and industrial funding. We have a forward and open-minded thinking and offer an ideal work surronding and well-equipped labs.

Web site for additional job details

Offer Requirements

Skills/Qualifications

The applicant (m/f/d) should have a profund background in biotechnology or bioprocess engineering. Ideally, the applicant have already experience with stem cells preferably mesenchymal stem cells or also iPSCs. Further, a strong interest in technology and applied sciences is a benefit.

Work location(s)
1 position(s) available at
Institute of Bioprocess Engineering and Pharmaceutical Technology (IBPT), University of Applied Sciences Mittelhessen (THM)
Germany
Giessen

EURAXESS offer ID: 609760

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