ORGANISATION/COMPANYTokyo Institute of Technology
RESEARCH FIELDAnthropologyBiological sciencesChemistryTechnology › Chemical technologyTechnology › Materials technologyTechnology › Micro-technologyTechnology › Nanotechnology
RESEARCHER PROFILERecognised Researcher (R2)
APPLICATION DEADLINE31/01/2021 23:59 - Asia/Tokyo
LOCATIONJapan › Tokyo, Kanagawa
TYPE OF CONTRACTTemporary
HOURS PER WEEKSee description
MEXT project “Genome Modality" Group: DNA Nanoscale Modality
Research Summary: DNA is a hierarchical soft matter that has a rigid structure at the nanoscale but has flexible macromolecular properties at the genomic DNA scale, where the genomic DNA scale is mesoscopic (hundreds of nm) to microscale (tens of micrometers)). While the nanoscale thermodynamics of DNA, governed by base sequences, is known, less is known about the mesoscopic scale and microscale structural properties and dynamics, essential for cell biology.
This project will elucidate the biophysics and soft matter physics principles that explain how DNA exhibits mesoscopic scale to microscale multimodal structures, properties, and functions of genomic DNA in a tiny space of the cell nucleus scale, based on nanoscale information. Here, we estimate the phenomena and properties such as liquid-liquid phase separation, intramolecular phase separation, interfacial tension, viscoelasticity, volume phase transition, and non-equilibrium, as the mesoscopic scale to microscale multimodal structures, properties, and functions. To construct genome DNA models (artificial chromatins), we use DNA gels and DNA droplets constructed with the self-assembly of DNA nano-motifs and DNA origami nanostructures. Also, to construct cell nuclear models (artificial cell nuclei), lipid membrane vesicles generated by microfluidics are used. We will apply findings in this study to biophysical and applied physics technologies, such as artificial cell engineering and molecular robotics.
This position is open to highly motivated postdoctoral researchers in DNA nanotechnology, nucleic acid chemistry, life sciences, biophysics, soft matter physics, biomolecular chemistry, MEMS, etc., who can engage in experimental research of the above research plan.
Construction of various shapes mesoscopic-sized DNA gel/DNA droplets (genome DNA models) by assembling DNA nanostructures. Measuring physical properties such as viscoelasticity and stability. Elucidation of the physicochemical mechanism of the genome DNA models using the knowledge of biophysics, soft matter physics, and polymer science. Functionalization of the genome DNA models using enzymes and other chemical reactions. Control of the physical properties of the genome DNA models using light and other methods. Besides, building a cell nuclear model to understand the interaction with the lipid membrane.
Sato et al., Science Advances (2020), doi.org/10.1126/sciadv.aba3471
Kasahara et al., APL Bioengineering (2020), doi.org/10.1063/1.5132929
Ishikawa et al., Angew. Chem. Int. Ed. (2019), doi.org/10.1002/anie.201908392
Kurokawa et al., Proc. Natl. Acad. Sci. USA (2017), doi.org/10.1073/pnas.1702208114
Tokyo Institute of Technology
J2 building, Suzukakedai Campus, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama City, Kanagawa 226-8502, Japan
[Job title and number of people]
One postdoctoral researcher
[Date of Appointment]
April 2021. The start date can be changed slightly before or after, on the applicants' request.
[Availability of Contract Renewal]
The contract renewal is considered based on the researcher's research progress, work performance, work attitude, and health status. Renewal will be done on an annual basis. However, the term of employment for this project is limited to March 31, 2025.
Flexible work hours, Full-time work
[Employment period/probationary period]
The contract may be renewed based on the researcher's research progress, work performance, work attitude, and health status. Renewal will be done on an annual basis. However, the term of employment for this project is limited to March 31, 2025. The probationary period shall be one month.
[Salary, Holidays, Vacation, Insurance]
Following the Tokyo Institute of Technology Rules of Employment for Certain Fixed-Term Employees.
EURAXESS offer ID: 590699
Posting organisation offer ID: D120121220
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