09/09/2019
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Short-term Early Stage Researcher (MSCA)

This job offer has expired


  • ORGANISATION/COMPANY
    Stazione Zoologica Anton Dohrn Napoli
  • RESEARCH FIELD
    Biological sciencesBiology
  • RESEARCHER PROFILE
    First Stage Researcher (R1)
  • APPLICATION DEADLINE
    23/09/2019 00:00 - Europe/Brussels
  • LOCATION
    Italy › Naples
  • TYPE OF CONTRACT
    Temporary
  • JOB STATUS
    Full-time
  • HOURS PER WEEK
    40
  • OFFER STARTING DATE
    01/10/2019
  • EU RESEARCH FRAMEWORK PROGRAMME
    H2020 / Marie Skłodowska-Curie Actions
  • REFERENCE NUMBER
    21/2019
  • MARIE CURIE GRANT AGREEMENT NUMBER
    766053

OFFER DESCRIPTION

Identification of piRNAs and microRNAs involved in sea urchin germ line specification

A 12 month short term fellowship is available under the supervision of Maria Ina Arnone at the Stazione Zoologica Anton Dohrn (SZN) in Napoli, Italy, within the Marie Curie ITN “EvoCELL” (https://www.evocell-itn.eu). The preferred starting date is 1st October 2019.

 

General information on the EvoCELL Network

EvoCELL is a Marie Skłodowska-Curie Innovative Training Network addressing the evolution of cell types using a diverse array of vertebrates and invertebrates. The network unites 8 academic and 2 non-academic organisations from 6 European countries. It provides a multi-disciplinary and international environment with training opportunities and exchange between the Network members. The labs in the network are using single-cell and tissue genomic techniques, merging them with more traditional approaches. All fellows will also contribute to scientific outreach and communication to a wider public as a fundamental part of the project. Applications are invited from excellent candidates seeking training in the field of animal evolution from a cell-type perspective. For general information on the EvoCELL short term fellowships see: https://euraxess.ec.europa.eu/jobs/377512

Project description

Main objective of this project will be the production of small RNA libraries from small micromere descendant dissociated cells in Strongylocentrotus purpuratus to search for and characterize piRNAs and microRNAs involved in germ line specification. The work will involve sea urchin germ cell isolation, library construction, sequencing and CRISPR-Cas9 mediated knock-out of key small micromere specific regulatory genes. Applicants should have some experience in molecular biology, developmental biology and/or genetics. Previous experience in sea urchin embryos and CRISPR-Cas9 mediated gene editing approaches would be preferred but are not required.

More Information

Eligibility criteria

Applicants should have a Masters degree or equivalent (e.g. a 5-year university degree) in a relevant field, but cannot already possess a doctoral degree. Experience in experimental molecular biology and bioinformatics is preferable. To be eligible for the fellowship, candidates should have no more than 4 years of research experience since graduation from a bachelor’s level degree. Candidates of all nationalities may apply, but they must not have lived in Italy for more than 12 months in the 3 years immediately prior to their appointment. The fellow will receive a competitive salary, including a mobility allowance and social security.

More information about the Arnone lab can be found at: http://www.szn.it/index.php/it/personale/staff/31-arnone-maria-i/694-arnone-maria-i

Selection process

- Applicants should apply as described at SZN website (http://www.szn.it/index.php/it/bandi-e-concorsi/bandi-di-concorso/assegni-di-ricerca/bandi-attivi). Informal enquiries (including a short letter of interest and CV) can be sent to miarnone@szn.it

Additional comments

- The starting date is flexible but would preferably be between 1st October and 1st November 2019.

Web site for additional job details

Offer Requirements

Skills/Qualifications

We seek an Early Stage Researcher (ESR) to study piRNAs and microRNAs in sea urchin germ line in the context of EvoCELL Marie Skłodowska-Curie Innovative training Network (www.evocell-itn.eu). Main objective of this ESR will be the production of small RNA libraries from small micromere descendant dissociated cells in Strongylocentrotus purpuratus to search for and characterize piRNAs and microRNAs involved in germ line specification. The work will involve sea urchin germ cell isolation, library construction, sequencing and CRISPR-Cas9 mediated knock-out of key small micromere specific regulatory genes.

Applicants should have some experience in molecular biology, developmental biology and/or genetics. Previous experience in sea urchin embryos and CRISPR-Cas9 mediated gene editing approaches would be preferred but are not required.

Work location(s)
1 position(s) available at
Stazione Zoologica Anton Dohrn Napoli
Italy
Naples
80121
Villa Comunale

EURAXESS offer ID: 444361

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