ORGANISATION/COMPANYUniversité Côte d'Azur
RESEARCH FIELDBiological sciences › BiologyComputer scienceMathematicsMedical sciences
RESEARCHER PROFILEFirst Stage Researcher (R1)
APPLICATION DEADLINE12/08/2019 17:00 - Europe/Brussels
LOCATIONFrance › Nice
TYPE OF CONTRACTTemporary
HOURS PER WEEK37
OFFER STARTING DATE15/11/2019
EU RESEARCH FRAMEWORK PROGRAMMEH2020 / Marie Skłodowska-Curie Actions COFUND
MARIE CURIE GRANT AGREEMENT NUMBER847581
Super-reolution Dynamic Imaginig of Intracellular Compartments in a Human Fungal Pathogen
Worldwide, fungal infections cause significant morbidity and mortality and Candida species are major etiological agents of such life-threatening infections. Candida albicans, a normally harmless commensal, is found on mucosal surfaces in most healthy individuals, yet it can cause superficial as well as life-threatening systemic infections. Its ability to switch from an ovoid to a filamentous form, in response to environmental cues, is critical for its pathogenicity.
The apical zone of the filament is densely packed with multiple highly dynamic membrane compartments, including secretory vesicles and Golgi cisternae. To understand the exquisite regulation of apical polarized growth, it is critical to follow the movement of these compartments in 3D, with high spatial and temporal resolution. This project will develop, optimize and apply super-resolution imaging approaches, in particular those taking advantage of fluorescent molecule blinking and their independent fluctuations in time, to study membrane traffic reorganization during filamentous growth in this Human fungal pathogen.
The recruited PhD student will follow different fluorescent protein fusions expressed in C. albicans live cells in super-resolved images obtained by reconstruction from wide-field acquisition. The entire acquisition pipeline will be optimized, from the experimental conditions to the reconstruction algorithm, for quantitative analysis of C. albicans hyphal, subcellular structure and dynamics.
The supervisors have extensive experience in image processing and reconstruction (L. Blanc-Féraud) and fungal cell biology (R. Arkowitz) and S. Schaub has developed a super-resolution microscope taking advantage of multiple-angle total internal reflection fluorescence.
The programme will provide excellent working conditions to the ESRs:
- Attractive salary of 2709,00 € (gross salary) per month, as well as, different allowances (between 815 and 1215 euros per month) ;
- The legal working time is 37 hours per week, with a daily working duration that does not exceed 10 hours ;
- Subsidized lunches and monthly pass for public transportation ;
- A total amount of yearly vacations of 45 days ;
- Paid sick leaves ;
- Parental leaves following the birth/adoption of a child ;
- Sick and parental leaves add up to the 42-month duration of the contract ;
- In addition to their income, the doctoral candidates who have family obligations will receive an extra family allowance of €400 per month. Furthermore, they will benefit for each child of a monthly financial help from the French social security (calculations based on the household income and on the number of children under the age of 20).
BoostUrCAreer will abide by the mobility rule defined for MSCA, the reference date being the call deadline, meaning applicants will only be eligible if they have lived or had their main activity (work, studies etc.) in France for less than 12 months within the past three years at the time of recruitment (November 15th).
Applicants must have a master’s degree or an international equivalent.
The selection process is detailed in the Applicant Guide available on the BoostUrCAreer application website.
Web site for additional job details
REQUIRED LANGUAGESENGLISH: Excellent
Candidates should have either a strong math/computational (convex/nonconvex sparse optimization in image processing, time series deconvolution, super-resolution) or a strong biological/microbiological (microscopy, mycology) background and be motivated to work in an interdisciplinary environment, with the possibility of short stays in life science biotechnology companies.
EURAXESS offer ID: 419622
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