07/06/2019
The Human Resources Strategy for Researchers

PhD position in Marine Biology M/F - Environment, biological clocks and biomineralization

This job offer has expired


  • ORGANISATION/COMPANY
    CNRS
  • RESEARCH FIELD
    Biological sciences
    Environmental science
  • RESEARCHER PROFILE
    First Stage Researcher (R1)
  • APPLICATION DEADLINE
    30/07/2019 23:59 - Europe/Brussels
  • LOCATION
    France › BANYULS SUR MER
  • TYPE OF CONTRACT
    Temporary
  • JOB STATUS
    Full-time
  • HOURS PER WEEK
    35
  • OFFER STARTING DATE
    02/09/2019

Workplace: Observatoire Océanologique de Banyuls (OOB), Avenue Pierre Fabre, 66650 Banyuls-sur-mer.

This PhD thesis set within an interdisciplinary collaboration between Laboratoire d'Ecogéochimie des Environnements Benthiques (LECOB), the laboratory of Biologie Intégrative des Organismes Marins (BIOM), the Laboratoire d'Océanographie Microbienne (LOMIC) and the Observatoire Océanologique de Banyuls (OOB).
Outside of high throughput sequencing, all the analytical material necessary for the successful completion of the thesis is available on site, thanks to the support of technology platforms in imaging (BIOPIC) and molecular biology (BIO2MAR) at the OOB.

The PhD grant is funded by the CNRS in the framework of its mission for transverse and interdisciplinary initiatives (MITI) project Prime|80 (TEMPO), which encourages and supports interdisciplinary projects between CNRS institutions.

The general aim of this thesis is to study the role of biological clocks on synchronization processes of biomineralization of the shell in the mussel M. galloprovincialis. The objective is to determine if the environment acts directly on the shell biomineralization process or if a biological clock allows the bivalve to anticipate the environmental circadian variation thus regulating the shell growth. We hypothesized that photoperiod (day/night alternation over a 24h cycle), temperature and/or trophic resources would synchronize the biological clock, alone or in combination, leading to control the rhythmic activity of shell biomineralization process in the Mediterranean mussel Mytilus galloprovincialis.
In order to achieve these objectives, the candidate will actively participate to different cruises on the Bay of Banyuls, dedicated to the collection of in situ data. He (she) will also be in charge of the implementation of the in vitro experiments at the OOB. Part of the work will be dedicated to the analysis of the growth patterns of the shell of M. galloprovincialis, obtained after chemical labelling techniques (calcein) and recapture. Sclerochronological analysis of thin sections of the shells will be reached by fluorescence microscopy and scanning electron microscopy (SEM). A transcriptome analysis of the main molecular players involved in biological clocks (clock genes such as Clock, Bmal, Cry, Per and Rorβ) and genes for metabolic activity and biomineralization (carbonic anhydrase, ATP-synthase, cytochrome oxidase, D lactate dehydrogenase), will be developed at different sampling points of the 24h cycle by a broadband approach (NanoString technology). The analysis of the amount of tissue biomarkers (lipids, proteins, carbohydrates) accessed by colorimetry will give a first indication of the assimilation of energy reserves, which will be complemented by the characterization of the variability of the food intake, approached by metabarcoding analysis of the stomach contents, achieved by high-throughput sequencing (Illumina MiSeq) of 16S and 18S rRNA genes.
Finally, the candidate will benefit from data of the Prime|80 project –TEMPO- dealing with the dynamic conditions and the variation of trophic resources potential in the mussel habitat, to carry out a comparative study of the different ecological compartments likely to initiate a rhythmic response of activity in this species.

Additional comments

Skills for this position include:
-ability for field work,
-good problem-solving skills,
-ability to work autonomously, team player,
-communication in English (written and oral),
-knowledge in statistics,
-experience in molecular biology and ecology of marine organisms are highly recommended.

Enquiries about the project and candidates' CV and cover letter can be sent to Franck Lartaud (franck.lartaud@obs-banyuls.fr) and Laurence Besseau (besseau@obs-banyuls.fr).

Web site for additional job details

Required Research Experiences

  • RESEARCH FIELD
    Biological sciences
  • YEARS OF RESEARCH EXPERIENCE
    None
  • RESEARCH FIELD
    Environmental science
  • YEARS OF RESEARCH EXPERIENCE
    None

Offer Requirements

  • REQUIRED EDUCATION LEVEL
    Biological sciences: Master Degree or equivalent
    Environmental science: Master Degree or equivalent
  • REQUIRED LANGUAGES
    FRENCH: Basic
Work location(s)
1 position(s) available at
Laboratoire d'Ecogéochimie des Environnements Benthiques
France
BANYULS SUR MER

EURAXESS offer ID: 415351
Posting organisation offer ID: 9949

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