RESEARCH FIELDBiological sciencesEnvironmental science
RESEARCHER PROFILEFirst Stage Researcher (R1)
APPLICATION DEADLINE30/07/2019 23:59 - Europe/Brussels
LOCATIONFrance › BANYULS SUR MER
TYPE OF CONTRACTTemporary
HOURS PER WEEK35
OFFER STARTING DATE02/09/2019
Workplace: Observatoire Océanologique de Banyuls (OOB), Avenue Pierre Fabre, 66650 Banyuls-sur-mer.
This PhD thesis set within an interdisciplinary collaboration between Laboratoire d'Ecogéochimie des Environnements Benthiques (LECOB), the laboratory of Biologie Intégrative des Organismes Marins (BIOM), the Laboratoire d'Océanographie Microbienne (LOMIC) and the Observatoire Océanologique de Banyuls (OOB).
Outside of high throughput sequencing, all the analytical material necessary for the successful completion of the thesis is available on site, thanks to the support of technology platforms in imaging (BIOPIC) and molecular biology (BIO2MAR) at the OOB.
The PhD grant is funded by the CNRS in the framework of its mission for transverse and interdisciplinary initiatives (MITI) project Prime|80 (TEMPO), which encourages and supports interdisciplinary projects between CNRS institutions.
The general aim of this thesis is to study the role of biological clocks on synchronization processes of biomineralization of the shell in the mussel M. galloprovincialis. The objective is to determine if the environment acts directly on the shell biomineralization process or if a biological clock allows the bivalve to anticipate the environmental circadian variation thus regulating the shell growth. We hypothesized that photoperiod (day/night alternation over a 24h cycle), temperature and/or trophic resources would synchronize the biological clock, alone or in combination, leading to control the rhythmic activity of shell biomineralization process in the Mediterranean mussel Mytilus galloprovincialis.
In order to achieve these objectives, the candidate will actively participate to different cruises on the Bay of Banyuls, dedicated to the collection of in situ data. He (she) will also be in charge of the implementation of the in vitro experiments at the OOB. Part of the work will be dedicated to the analysis of the growth patterns of the shell of M. galloprovincialis, obtained after chemical labelling techniques (calcein) and recapture. Sclerochronological analysis of thin sections of the shells will be reached by fluorescence microscopy and scanning electron microscopy (SEM). A transcriptome analysis of the main molecular players involved in biological clocks (clock genes such as Clock, Bmal, Cry, Per and Rorβ) and genes for metabolic activity and biomineralization (carbonic anhydrase, ATP-synthase, cytochrome oxidase, D lactate dehydrogenase), will be developed at different sampling points of the 24h cycle by a broadband approach (NanoString technology). The analysis of the amount of tissue biomarkers (lipids, proteins, carbohydrates) accessed by colorimetry will give a first indication of the assimilation of energy reserves, which will be complemented by the characterization of the variability of the food intake, approached by metabarcoding analysis of the stomach contents, achieved by high-throughput sequencing (Illumina MiSeq) of 16S and 18S rRNA genes.
Finally, the candidate will benefit from data of the Prime|80 project –TEMPO- dealing with the dynamic conditions and the variation of trophic resources potential in the mussel habitat, to carry out a comparative study of the different ecological compartments likely to initiate a rhythmic response of activity in this species.
Skills for this position include:
-ability for field work,
-good problem-solving skills,
-ability to work autonomously, team player,
-communication in English (written and oral),
-knowledge in statistics,
-experience in molecular biology and ecology of marine organisms are highly recommended.
Web site for additional job details
Required Research Experiences
RESEARCH FIELDBiological sciences
YEARS OF RESEARCH EXPERIENCENone
RESEARCH FIELDEnvironmental science
YEARS OF RESEARCH EXPERIENCENone
REQUIRED EDUCATION LEVELBiological sciences: Master Degree or equivalentEnvironmental science: Master Degree or equivalent
REQUIRED LANGUAGESFRENCH: Basic
EURAXESS offer ID: 415351
Posting organisation offer ID: 9949
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