11/10/2018
The Human Resources Strategy for Researchers

PhD project "Characterization of Arabidopsis mitochondria translation machinery"

This job offer has expired


  • ORGANISATION/COMPANY
    CNRS
  • RESEARCH FIELD
    Biological sciencesBotany
    TechnologyBiotechnology
  • RESEARCHER PROFILE
    First Stage Researcher (R1)
  • APPLICATION DEADLINE
    01/11/2018 23:59 - Europe/Brussels
  • LOCATION
    France › STRASBOURG
  • TYPE OF CONTRACT
    Temporary
  • JOB STATUS
    Full-time
  • HOURS PER WEEK
    35
  • OFFER STARTING DATE
    15/11/2018

Feasibility over 3 years
The project described here is based on strong preliminary data. Our team is a leader in the field of PPR proteins, in particular to study their involvement in translation and benefits from collaborative work with experts in this field. Finally, the project will benefit from all the necessary infrastructures available at the IBMP, i.e. greenhouses, growth cabinets and technical platforms such as Illumina sequencing, protein expression and purification, quantitative PCR and mass spectrometry. The project should thus be feasible in a time frame of 3 years.

PhD student supervision
For this work, the PhD student will first be supervised at full time until he gains sufficient autonomy. The group working on PPR protein, including the ones involved in translation, is reinforced by statutory researchers, post-docs and an engineer that will assist the PhD student in his every day work. The PhD student will also benefit from synergetic work within IBMP where many teams investigate gene expression processes in plants, in particular, translation.

Wished skills
The PhD student will be motivated and hard working. He will have a Master degree or its equivalent in biological sciences and will have a strong theoretical background in molecular biology and / or biochemistry. Particularly, we would be interested in students having gained experience in one or more of these topics: RNA binding proteins, plant genetics, immune-precipitations, deep sequencing and RNA seq data analysis.

Expertises which will be acquired during the training
The 3 years PhD training period will allow the student to become an autonomous scientific worker. He will have used a large number of genetic, molecular and biochemical methods. Beyond the technical training, the PhD student will have gained autonomy in the planning and design of experiments and in the analysis of their results. Writing expertise will also be gained. Beyond the PhD thesis itself, the student will also be extensively involved in writing his own results in scientific publications. Altogether, through research, the student will train creativity and independent thinking skills.

Characterization of Arabidopsis mitochondria translation machinery

Mitochondria are responsible for energy production through aerobic respiration and represent the powerhouse of eukaryotic cells. Their metabolism and gene expression processes combine bacterial-like features and traits that evolved in eukaryotes. Among mitochondrial gene expression processes, translation remains the most elusive. In plants, while numerous pentatricopeptide repeat (PPR) proteins are involved in all steps of gene expression, their function in translation remained unclear.
The biochemical characterisation of Arabidopsis mitochondrial ribosomes (mitoribosomes) performed in our laboratory has identify their protein subunit composition. Complementary biochemical approaches have identify 24 plant specific mitoribosome proteins, among which 12 are PPR proteins. Other preliminary date obtained by single particle cryo-electron microscopy suggest that Arabidopsis mitoribosomes are completely distinct from bacterial and other eukaryote mitoribosomes.
This thesis project will consist in the functional characterization of these novel PPR proteins specifically associated to plant mitoribosomes. Their function will be assessed by a combination of complementary approaches including classical reverse genetics, biochemistry, physiology, molecular biology and structural biology. For instance, the exact involvement of these proteins in translation will be analyzed by ribosome profiling experiments, i.e. through the purification of ribosome footprints obtained from control and PPR mutant plants followed by high throughput RNA sequencing.
Altogether, this project should reveal novel functions for PPR proteins and should help to understand the diversity and specialization of translation systems across eukaryotes.

Required Research Experiences

  • RESEARCH FIELD
    Biological sciencesBotany
  • YEARS OF RESEARCH EXPERIENCE
    None
  • RESEARCH FIELD
    Biological sciences
  • YEARS OF RESEARCH EXPERIENCE
    None
  • RESEARCH FIELD
    TechnologyBiotechnology
  • YEARS OF RESEARCH EXPERIENCE
    None

Offer Requirements

  • REQUIRED EDUCATION LEVEL
    Biological sciences: Master Degree or equivalent
    Technology: Master Degree or equivalent
  • REQUIRED LANGUAGES
    FRENCH: Basic
Work location(s)
1 position(s) available at
Institut de biologie moléculaire des plantes
France
STRASBOURG

EURAXESS offer ID: 346238
Posting organisation offer ID: 5848

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