ORGANISATION/COMPANYCenter for Physiopathology Toulouse-Purpan, INSERM UMR1043
RESEARCH FIELDBiological sciences › BiologyBiological sciences › Laboratory animal science
RESEARCHER PROFILEFirst Stage Researcher (R1)Recognised Researcher (R2)
APPLICATION DEADLINE30/03/2018 23:00 - Europe/Brussels
LOCATIONFrance › Toulouse
TYPE OF CONTRACTTemporary
HOURS PER WEEK40
OFFER STARTING DATE01/06/2018
IS THE JOB RELATED TO STAFF POSITION WITHIN A RESEARCH INFRASTRUCTURE?Yes
Job title: Research assistant in molecular biology
Location: Center for Physiopathology Toulouse-Purpan (Inserm-U1043/CNRS-U282), Toulouse, France
Contract: 7months+2 years, full-time
Starting date: June 2018
Salary: Competitive with benefits (1522-1860€ gross salary/month, subject to skills and experience).
Post-transcriptional regulation by RNA-binding proteins (RBPs) controls all aspects of RNA life, from synthesis, editing, location and translation into functionally active proteins. Using next-generation sequencing technologies we have uncovered novel RBP-dependent regulatory mechanisms that control innate and adaptive immune responses. In particular, we are interested to understand further how RBPs regulate gene expression, lymphocyte activation, selection and differentiation. We have a deep interest on uncovering the role of RBPs in controlled mutagenesis and DNA damage repair, two essential mechanisms for antibody repertoire expansion and life-long protection against infections.
The successful applicant is expected to help and support other team members in their day-to day tasks as well as help on managing mouse colonies. Development of own projects is possible under supervision based on candidate’s experience and desire for future career development. Excellent laboratory practices, high degree of organization, data analysis & recording and communication are essential. A positive aptitude for training and development of early researchers and students is also desired.
We are a young- group of enthusiastic scientists dedicated to uncover the fundamental biological processes that regulate gene expression in the immune system and in cancer. We are strongly committed to individual career development of all members of the team. We are based at the Center for Physiopathology Toulouse-Purpan (Inserm U1043; CNRS U5282, http://www.cptp.inserm.fr) with excellent FACs, imaging and animal facilities. We’re part of the University of Toulouse III, Oncopole and Genotoul.
We want to know about you if you are a recently qualified or experience technician/research assistant. Previous lab experience in RNA biology (e.g. RNA/DNA isolation, PCR), Biochemistry (e.g. vector cloning, blotting) and Immunology (e.g. flow cytometry, ELISAs, cell culture) is desired. Experience with mouse colony and general laboratory management will be highly appreciated. Evidence of aptitude for teamwork in science is essential. Computing skills and proficiency in English are needed.
Please send a cover letter and a CV (max. three A4 pages including your scientific contributions) describing your interests in Biomedical Research and Immunology as well as previous experience. At least two reference letters will be requested at a later stage. Address both informal and formal application to Dr. Manuel D. Diaz-Munoz (firstname.lastname@example.org). Deadline for applying: 30 March 2018. Interview in April 2018.
1.- RNA binding proteins control gene expression and cell fate in the immune system. Turner M & Díaz-Muñoz MD. Nature Immunology. 2018 Jan 18. doi:10.1038/s41590-017-0028-4.
2.- Tia1 dependent regulation of mRNA subcellular location and translation controls p53 expression in B cells. Díaz-Muñoz MD, et al. Nature Communications. 2017 Sep 13;8(1):530.
3.- The RNA-binding protein TTP is a global post-transcriptional regulator of feedback control in inflammation. Tiedje C, et al. Nucleic Acids Research. 2016 Sep 6;44(15):7418-40.
4.- RNA-binding proteins ZFP36L1 and ZFP36L2 promote cell quiescence. Galloway A, et al. Science. 2016 Apr 22;352(6284):453-9.
5.- The RNA-binding protein HuR is essential for the B cell antibody response. Diaz-Muñoz MD, et al. Nature Immunology. 2015 Apr;16(4):415-25.
Required Research Experiences
RESEARCH FIELDBiological sciences › Laboratory animal science
YEARS OF RESEARCH EXPERIENCE1 - 4
RESEARCH FIELDBiological sciences › Biology
YEARS OF RESEARCH EXPERIENCE1 - 4
REQUIRED EDUCATION LEVELBiological sciences: Master Degree or equivalent
REQUIRED LANGUAGESENGLISH: GoodFRENCH: Excellent
Research Assistant - Niveau BTS, DUT (at least 1-2 years experience. No more than 3 years)
Previous lab experience in RNA biology (e.g. RNA/DNA isolation, PCR), Biochemistry (e.g. vector cloning, blotting) and Immunology (e.g. flow cytometry, ELISAs, cell culture). Experience with mouse colony and general laboratory management. Evidence of aptitude for teamwork in science. Excellent laboratory practices, high degree of organization, data analysis & recording and communication. Positive aptitude for training and development of early researchers and students. Computing skills and proficiency in English are needed.
EURAXESS offer ID: 279032