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EURAXESS

1 funded PhD - study of microRNA expression profile in Central Areolar Chroidal Dystrophy

10 May 2023

Job Information

Organisation/Company
Universidad de Alicante
Department
Department of Physiology, Genetics and Microbiology
Research Field
Biological sciences
Chemistry
Neurosciences » Neurobiology
Biological sciences » Laboratory animal science
Biological sciences » Biological engineering
Researcher Profile
First Stage Researcher (R1)
Country
Spain
Application Deadline
Type of Contract
Temporary
Job Status
Full-time
Offer Starting Date
Is the job funded through the EU Research Framework Programme?
HE / MSCA
Reference Number
4
Marie Curie Grant Agreement Number
101073316
Is the Job related to staff position within a Research Infrastructure?
No

Offer Description

1 Doctoral Candidate position (DC4) at the Universidad de Alicante (UA) in Alicante, Spain is available in the framework of the Marie Skłodowska Curie - Doctoral Networks (MSCA-DN) project “Targeting RNA as an approach for treating retinal disease” (RETORNA). 

RETORNA proposes breakthrough research, distributed in 10 individual projects, performed by 10 Doctoral candidates (one of them being the DC4), in the following countries: IrelandSpainNetherlandsTürkiyeGermany and Italy.  DC4 will be hired by UA and working mainly in Alicante, with various secondments in RETORNA consortium.

Get to know the lab at UA:

Our lab focuses on the study of the morphology and physiology of the vertebrate retina in healthy and pathological conditions, using mice models and human tissue. We are especially interested in the study of the retinal remodeling that occurs during neurodegenerative diseases such as retinitis pigmentosa or central areolar choroidal dystrophy, and in the search of possible therapies to prevent, reverse, or slow retinal degeneration. For that, we use a wide variety of techniques to assess retinal histology (immunohistochemistry, confocal microscopy, electron transmission microscopy), visual behavioral (optomotor test) and electrophysiological response (electroretinogram), or molecular biology (western blot, qPCR). 

For more information about the research group: https://retinalmicroscopy.com;

Neurobiología del sistema visual y terapia de enfermedades neurodegenerativas (NEUROVIS)

 

RETORNA summary description:

RETORNA aims to treat retinal diseases (RD) by using RNA therapy. Among the research objectives, we will study different types of RNA that are expressed in retinal cells. By observing bioinformatic models, cell and tissue cultures, animal models, and human patients, we will be able to create RNA profiles for different RD. This screening will identify several targets that will be used as therapeutic approaches. Then, selected RNAs will be used to treat different RD, such as macular degeneration and retinitis pigmentosa.

The proposed doctoral network (DN) will provide an innovative, international, multi/interdisciplinary platform to train young scientists in the field of vision science. 

For more information about RETORNA: https://cordis.europa.eu/project/id/101073316

 

Please read carefully ALL the details of the individual project DC4 and apply only if really interested!

Title: Study of microRNA expression profile in Central Areolar Choroidal Dystrophy. Translational study using a new CRISPR-generated animal model.

Host supervisor: Dr. Nicolás Cuenca and Dr. Natalia Martínez (Universidad de Alicante, Spain)

Objectives: Central Areolar Choroidal Dystrophy (CACD) is a monogenic hereditary retinal dystrophy that involves a progressive retinal degeneration. It usually causes a gradual loss of visual acuity between 30 and 60 years old, and color perception impairments. In most cases, CACD presents an autosomal dominant inheritance pattern in which patients carry a single mutation in the peripherin gene (PRPH2 or RDS, Retinal Degeneration Slow), although other genes may be involved in some individuals. Peripherin is a glycoprotein essential for generating and maintaining the photoreceptor outer segment disks where retinal phototransduction takes place. Five different mutations in peripherin have been histologically and functionally described as causing this disease and this project will focus on the p.Arg195Leu mutation which was firstly described in a carrier family in Japan and later in German and Spanish families. In Spain, CACD has been diagnosed in a large family from the Iguña Valley, Cantabria. The existence of this family, with a large number of members affected with the same mutation, is a unique opportunity to in-depth study the CACD pathology in a homogeneous population. Thus, our new CRISPRgenerated animal model (Prph2 KI/WT mouse), that reproduces the same pathological characteristics than CACD patients, will allow a
better description of the disease and increase the current knowledge about it. The main aim of this project is to study for the first time the total microRNA expression profile in the retina of Prph2 KI/WT mouse and particularly focusing on the miRNAs which have an important role in the maintenance and function of photoreceptor outer segments (e.g miR-183/96/182 cluster) and also on those miRNAs associated with inflammatory pathways (e.g. miR-155 and miR-124). In addition, a study of retinal morphology and physiology will be carried out to describe the neuropathological events that occur in CACD patients and in this new mouse model carrying the same mutation. This study will allow to test new neuroprotective therapies using RNA-based strategies in Prph2 KI/WT mice, as a first approach for translational medicine.

Expected Results:
1. Analysis of retinal morphology and physiology to describe the neuropathological events that occur in CACD patients and in this new mouse model carrying the same mutation.
2. Assessment the global expression profile of mRNAs and miRNAs in Prph2 KI/WT mouse across the progression of the retinal neurodegenerative process. To establish a correlation between the miR-183/96/182 cluster and their relationship with peripherin mutation.
3. In silico identification of key pathways regulated by the RNAs and miRNAs that are modified by the peripherin mutation and specifically miR-155 and miR-124.
4. Evaluation of a possible therapy for CACD using RNA-based strategies in Prph2 KI/WT mouse.

Secondments are foreseen during the 36 months project.

Requirements

Research Field
All
Education Level
Master Degree or equivalent
Skills/Qualifications

Candidates reseachers must:

  • have MSc level training in the field of Biological Sciences, Biotechnology, Neurosciences, Biomedicine or similar related to the project
  • possess scientific skills and research experience related to the DC4 project
  • desired skills
  1. Good experience with animal handling.
  2. Basic knowledge of bioinformatic tools for RNA data analysis.
  • possess a good working level of English: candidates must demonstrate during the interview a good ability to understand and express themselves in both written and spoken English, sufficiently high to fully benefit from the experience 
  • be highly motivated to participate in the 3 year programme and commit to obtain the PhD degree
  • be creative and possess a high level of independency 
  • be available and agree with travelling in the RETORNA consortium for secondments
Languages
ENGLISH
Level
Good
Research Field
AllAll

Additional Information

Benefits

RETORNA Doctoral candidate  will be employed according to the rules for Doctoral Candidates in MSCA Doctoral Network and the general regulations of the hosting institution.

The monthly financial package will include the following researcher allowances:

1) Living allowance 

2) Mobility allowance 

3) Family allowance if applicable.

The full-time gross yearly salary amounts to € 36.026,00 Euro (family allowance will be calculated only if applicable).

The guaranteed PhD funding is for 36 months starting from autumn 2023.

 

Other benefits:

The selected candidate, together with other 9 DCs that will be selected, will benefit from a large variety of activities foreseen for them. DCs at RETORNA will be provided with a unique multi-disciplinary and multi-sectoral training. First and foremost, DCs will be trained as scientists, each of them in their host institution, at their research secondments, and through local and network wide events. Next, thanks to the partnership between academic and non-academic entities, DCs will receive multi-sectoral inputs from both worlds. This will happen via lectures and workshops in our varied training program.

 

 

    Eligibility criteria

    Please read carefully ALL the eligibility criteria and apply only if you possess them:

    • candidates can be of any nationality
    • must comply with the following mobility rule: researchers are required to undertake trans-national mobility (i.e., move from one country to another) and participate in all the activities foreseen by the project when taking up the appointment (trainings, summer courses, annual meetings, lectures, participation in conferences, public activities).
    • possess a Master degree or an equivalent that allows the access to the doctoral program in the Universidad de Alicante 
    • be, at the date of recruitment, a doctoral candidate (i.e. not already in possession of a doctoral degree). Researchers who have successfully defended their doctoral thesis but who have not yet formally been awarded the doctoral degree will not be considered eligible.
    • not have resided or carried out their main activity (work, studies, etc.) in Spain for more than 12 months in the 3 years immediately before the recruitment date. Short stays, such as holidays, are not taken into account.

     

    Selection process

    The RETORNA selection procedure is open, transparent, merit-based and in line with the Code of Conduct for the Recruitment of Researchers and the European Charter for Researchers.

    The criteria for recruitment of DCs will respect the principles of non-discrimination contained in The Charter of Fundamental Rights of the European Union.

    It is the policy of the RETORNA project to promote and give equal opportunities regardless of gender and stimulate gender balance.

     

    Documents required 

    Interested candidates should submit the following documents via email to andreea.craciun@ucv.es, indicating in the e-mail title "RETORNA - DC4 application":

    • a detailed CV, including – if applicable – relevant publications
    • a motivation letter, indicating why you wish to conduct this research project
    • two references
    • transcripts of bachelor and master degrees
    • English certificate if any

    All documents must be named as "type of document_Name Surname" (i.e. Motivation letter_Andreea Craciun")

    We encourage candidates to send any other certificates, representative publications, academic references or links demostrating the experience and interest in the project. 

     

    Selection process

    The selection process will foreseen the following steps:

    1.  Eligibility: the RETORNA recruitment committee will check that each application is complete and that applicants fulfil the eligibility criteria described in the previous section.

    2. Evaluation: each eligible application will be assessed by the principal investigators of our network. The principal investigator will select up to four candidates per position for the following steps:

    a. Online interviews: online interviews will be scheduled, in person interview will be organized where possible. Short-listed candidates will be invited for an online interview with the supervisor responsible for the research project. At least one member of the recruitment committee will attend the online interviews.

    b. Selection results: our recruitment committee will notify short-listed candidates of the results.

    c. Hiring process and enrollment: the selected candidates will be put in touch with the principal investigator and the HR Department of the Universidad de Alicante to initiate the hiring procedure.

    Applicants who have not been successful but who have received a positive evaluation will be put on a waiting list to cover possible withdrawals and future positions.

     

    Recruitment calendar:

    • Deadline for applications: 24th of May 
    • Eligibility and Evaluation: 25-26th of May  2023 
    • Online Interviews: 29 May - 6 of June 2023
    • Notification to candidates: 7th of June 2023
    • Hiring process: June - August 2023
    • Start date of the PhD:  September - October 2023
    Additional comments

     

     

      Work Location(s)

      Number of offers available
      1
      Company/Institute
      Universidad de Alicante
      Country
      Spain
      State/Province
      Comunidad Valenciana
      City
      Alicante
      Postal Code
      03690
      Street
      San Vicente Raspeig
      Geofield

      Where to apply

      E-mail
      andreea.craciun@ucv.es

      Contact

      State/Province
      Spain
      City
      Alicante
      Website
      Street
      San Vicente Raspeig
      Postal Code
      03690