The BILAT USA 2.0 guide is intended to help US researchers and innovators find and take advantage of opportunities to collaborate with European colleagues in Horizon 2020, the Eighth Framework Programme for Research and Innovation of the European Union. The guide describes the program architecture, identifies specific opportunities for US researchers, explains specific rules and regulations for participation and funding in Europe, how to prepare an application, negotiate grants and consortium agreements, manage a project and protect intellectual property rights.